Article
Disruption of Yarrowia lipolytica TPS1 gene encoding trehalose-6-P synthase does not affect growth in glucose but impairs growth at high temperature.
PloS one - 1 Jan 2011
Flores Carmen-Lisset, Gancedo Carlos, Petit Thomas
Abstract excerpt
We have cloned the Yarrowia lipolytica TPS1 gene encoding trehalose-6-P synthase by complementation of the lack of growth in glucose of a Saccharomyces cerevisiae tps1 mutant. Disruption of YlTPS1 could only be achieved with a cassette placed in the 3' half of its coding region due to the overlap of its sequence with the promoter of the essential gene YlTFC1. The Yltps1 mutant grew in glucose although the Y....
Topics
- Chromosomes, Fungal
- Cloning, Molecular
- Glucose
- Glucosyltransferases
- Heat-Shock Response
- Molecular Sequence Data
- Mutation
- Spores, Fungal
- Temperature
- Trehalase
- Trehalose