Investigation of methylation at Hha I sites using the hypervariable probe M27 beta allows improved clonal analysis in myeloid leukaemia and demonstrates differences in methylation between leukaemic and remission samples.

Abstract excerpt

The methylation-sensitive enzyme Hha I has been used to assess the differentially methylated patterns on active and inactive X-chromosomes at the DXS255 locus recognized by the hypervariable probe M27 beta. The X-chromosome inactivation ratios obtained from 37 haematologically normal females using PstI and HhaI and correlated well with results obtained using PstI Hpa II (r = 0.97), and in 19 individuals with...

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