Article
A fibroblast glutaryl-CoA dehydrogenase assay using detritiation of 3H-labelled glutaryl-CoA: application in the genotyping of the glutaryl-CoA dehydrogenase locus.
Clinica chimica acta; international journal of clinical chemistry - 29 Oct 1993
Christensen E
Abstract excerpt
A method described earlier for measuring glutaryl-CoA dehydrogenase activity in fibroblasts has been further developed. This assay uses the detritiation of [2,3,4-3H]glutaryl-CoA both with and without added artificial electron acceptors as a measure of glutaryl-CoA dehydrogenase activity. Fibrobl...
Topics
- Cells, Cultured
- Fibroblasts
- Genotype
- Glutarates
- Glutaryl-CoA Dehydrogenase
- Humans
- Oxidoreductases
- Oxidoreductases Acting on CH-CH Group Donors
- Tritium