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Article

A covalent enzyme-substrate intermediate with saccharide distortion in a mutant T4 lysozyme.

Science (New York, N.Y.) - 24 Dec 1993

Kuroki R, Weaver L H, Matthews B W

Abstract excerpt

The glycosyl-enzyme intermediate in lysozyme action has long been considered to be an oxocarbonium ion, although precedent from other glycosidases and theoretical considerations suggest it should be a covalent enzyme-substrate adduct. The mutation of threonine 26 to glutamic acid in the active site cleft of phage T4 lysozyme (T4L) produced an enzyme that cleaved the cell wall of Escherichia coli but left the...

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PMID: 8266098
DOI: 10.1126/science.8266098

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