Article
A covalent enzyme-substrate intermediate with saccharide distortion in a mutant T4 lysozyme.
Science (New York, N.Y.) - 24 Dec 1993
Kuroki R, Weaver L H, Matthews B W
Abstract excerpt
The glycosyl-enzyme intermediate in lysozyme action has long been considered to be an oxocarbonium ion, although precedent from other glycosidases and theoretical considerations suggest it should be a covalent enzyme-substrate adduct. The mutation of threonine 26 to glutamic acid in the active si...
Topics
- Amino Acid Sequence
- Animals
- Bacteriophage T4
- Binding Sites
- Carbohydrate Conformation
- Carbohydrate Sequence
- Cell Wall
- Chickens
- Disaccharides
- Egg White
- Escherichia coli
- Molecular Sequence Data
- Muramidase
- Mutation
- Oligopeptides
- Peptidoglycan