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Article

Catalysis by hen egg-white lysozyme proceeds via a covalent intermediate.

Nature - 23 Aug 2001

Vocadlo D J, Davies G J, Laine R, Withers S G

Abstract excerpt

Hen egg-white lysozyme (HEWL) was the first enzyme to have its three-dimensional structure determined by X-ray diffraction techniques. A catalytic mechanism, featuring a long-lived oxocarbenium-ion intermediate, was proposed on the basis of model-building studies. The 'Phillips' mechanism is widely held as the paradigm for the catalytic mechanism of beta-glycosidases that cleave glycosidic linkages with net...

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PMID: 11518970
DOI: 10.1038/35090602

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

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