Article
Conformational shifts propagate from the oligomerization domain of p53 to its tetrameric DNA binding domain and restore DNA binding to select p53 mutants.
The EMBO journal - 15 Dec 1993
Halazonetis T D, Kandil A N
Abstract excerpt
p53 is a conformationally flexible sequence-specific DNA binding protein mutated in many human tumors. To understand why the mutant p53 proteins associated with human tumors fail to bind DNA, we mapped the DNA binding domain of wild-type p53 and examined its regulation by changes in the protein c...
Topics
- Amino Acid Sequence
- Binding Sites
- DNA-Binding Proteins
- Genes, p53
- Humans
- In Vitro Techniques
- Macromolecular Substances
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides
- Protein Binding
- Protein Conformation
- Structure-Activity Relationship
- Tumor Suppressor Protein p53