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Article

Cloning of flagellar genes in Chlamydomonas reinhardtii by DNA insertional mutagenesis.

Genetics - 1 Oct 1993

Tam L W, Lefebvre P A

Abstract excerpt

Chlamydomonas is a popular genetic model system for studying many cellular processes. In this report, we describe a new approach to isolate Chlamydomonas genes using the cloned nitrate reductase gene (NIT1) as an insertional mutagen. A linearized plasmid containing the NIT1 gene was introduced into nit1 mutant cells by glass-bead transformation. Of 3000 Nit+ transformants examined, 74 showed motility defects of a...

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Identifiers

PMID: 8244002
DOI: 10.1093/genetics/135.2.375
PMCID: PMC1205643

Genes

NIT1

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Source receipt: pubmed, retrieved 7 Sept 2026.

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