Article
Cloning of flagellar genes in Chlamydomonas reinhardtii by DNA insertional mutagenesis.
Genetics - 1 Oct 1993
Tam L W, Lefebvre P A
Abstract excerpt
Chlamydomonas is a popular genetic model system for studying many cellular processes. In this report, we describe a new approach to isolate Chlamydomonas genes using the cloned nitrate reductase gene (NIT1) as an insertional mutagen. A linearized plasmid containing the NIT1 gene was introduced in...
Topics
- Alleles
- Animals
- Cell Movement
- Chlamydomonas reinhardtii
- Cloning, Molecular
- Crosses, Genetic
- DNA, Fungal
- Flagella
- Genes, Fungal
- Mutagenesis, Insertional
- Nitrate Reductase
- Nitrate Reductases
- Phenotype
- Plasmids
- Restriction Mapping
- Transformation, Genetic