Rescue of a paralyzed-flagella mutant of Chlamydomonas by transformation.

doi:10.1073/pnas.87.15.5739

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Article

Rescue of a paralyzed-flagella mutant of Chlamydomonas by transformation.

Proceedings of the National Academy of Sciences of the United States of America - 1 Aug 1990

Diener D R, Curry A M, Johnson K A, Williams B D, Lefebvre P A, Kindle K L, Rosenbaum J L

Abstract excerpt

The biflagellate alga Chlamydomonas has been used extensively in the genetic and biochemical analysis of flagellar assembly and motility. We have restored motility to a paralyzed-flagella mutant of Chlamydomonas by transforming with the corresponding wild-type gene. A nitrate reductase-deficient...

Topics

Blotting, Southern
Cell Movement
Chlamydomonas
DNA
Electrophoresis, Gel, Two-Dimensional
Flagella
Mutation
Phenotype
Plant Proteins
Plasmids
Transformation, Genetic

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