Genetic and genomic approaches to identify genes involved in flagellar assembly in Chlamydomonas reinhardtii.

doi:10.1016/bs.mcb.2014.12.001

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Article

Genetic and genomic approaches to identify genes involved in flagellar assembly in Chlamydomonas reinhardtii.

Methods in cell biology - 1 Jan 2015

Lin Huawen, Dutcher Susan K

Abstract excerpt

Flagellar assembly requires intraflagellar transport of components from the cell body to the flagellar tip for assembly. The understanding of flagellar assembly has been aided by the ease of biochemistry and the availability of mutants in the unicellular green alga, Chlamydomonas reinhardtii. In this chapter, we discuss means to identify genes involved in these processes using forward and reverse genetics. In...

Topics

Base Sequence
Chlamydomonas reinhardtii
Chromosome Mapping
DNA, Plant
Flagella
Genome, Plant
Molecular Sequence Data
Mutagenesis, Insertional
Polymorphism, Single Nucleotide
Protein Transport
Sequence Analysis, DNA
Transcriptome

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Identifiers

PMID: 25837400
DOI: 10.1016/bs.mcb.2014.12.001
PMCID: PMC5777853

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