Article
Determinants of the brain-specific expression of the rat aldolase C gene: ex vivo and in vivo analysis.
European journal of biochemistry - 15 Nov 1993
Thomas M, Makeh I, Briand P, Kahn A, Skala H
Abstract excerpt
A 115-bp promoter fragment of the aldolase C gene is sufficient for conferring neural cell specificity on a reporter gene, in cultured PC12 cells and in transgenic mice. In vitro DNase I protection experiments detected two footprints on the promoter, termed boxes A/A', and B. The 5' A/A' box contains overlapping Sp1 and Krox20/Krox24 binding sites; it binds Sp1 in fibroblasts (box A') and a different complex in...
Topics
- 3T3 Cells
- Animals
- Base Sequence
- Binding Sites
- Brain
- Chloramphenicol O-Acetyltransferase
- DNA
- DNA-Binding Proteins
- Deoxyribonuclease I
- Fructose-Bisphosphate Aldolase
- Gene Expression Regulation, Enzymologic