Purification and immunological characterization of acid beta-galactosidase from dog liver.

doi:10.1016/0305-0491(93)90315-v

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Article

Purification and immunological characterization of acid beta-galactosidase from dog liver.

Comparative biochemistry and physiology. B, Comparative biochemistry - 1 Oct 1993

Hotamisligil S, Hale S, Alroy J, Fischer I, Raghavan S

Abstract excerpt

1. Dog liver acid beta-galactosidase was isolated in high yield and purified to homogeneity using a series of chromatographies on Con A-Sepharose, decyl-agarose, anion-exchange HPLC and gel-filtration HPLC. 2. Non-denaturing gel filtration by HPLC gave a single homogeneous peak corresponding to m...

Topics

Animals
Antibody Specificity
Blotting, Western
Chromatography, High Pressure Liquid
Cross Reactions
Dogs
Humans
Liver
Molecular Weight
Mutation
Species Specificity
beta-Galactosidase

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