Article
Purification and immunological characterization of acid beta-galactosidase from dog liver.
Comparative biochemistry and physiology. B, Comparative biochemistry - 1 Oct 1993
Hotamisligil S, Hale S, Alroy J, Fischer I, Raghavan S
Abstract excerpt
1. Dog liver acid beta-galactosidase was isolated in high yield and purified to homogeneity using a series of chromatographies on Con A-Sepharose, decyl-agarose, anion-exchange HPLC and gel-filtration HPLC. 2. Non-denaturing gel filtration by HPLC gave a single homogeneous peak corresponding to molecular mass of 180-190 kDa. During SDS-PAGE analysis, the single peak dissociated into a major band corresponding to...
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