Article
Cre-loxP-mediated gene replacement: a mouse strain producing humanized antibodies.
Current biology : CB - 1 Dec 1994
Zou Y R, Müller W, Gu H, Rajewsky K
Abstract excerpt
BACKGROUND: The bacteriophage-derived Cre-loxP recombination system operates efficiently in mammalian cells. This system is particularly useful in gene-targeting experiments in the mouse, and has already been used to generate 'clean' deletions of target genes in the germ line, as well as to inactivate target genes in a conditional manner (based on regulated expression of the Cre recombinase). In principle,...
Topics
- Animals
- Base Sequence
- Cells, Cultured
- DNA Nucleotidyltransferases
- DNA Primers
- Gene Expression
- Gene Targeting
- Humans
- Immunoglobulin Constant Regions
- Immunoglobulin G
- Immunoglobulin Heavy Chains