[A second gene involved in the formation of disulfide bonds in proteins localized in Escherichia coli periplasmic space].

Abstract excerpt

A novel mutant of Escherichia coli was isolated whose phenotype is similar to those of dsbA strains. For instance, it is unable to express pH 2.5 acid phosphatase, glucose-1-phosphatase and alkaline phosphatase in the periplasmic space. The mutation lies at min 26.2 of the linkage map, and does not affect expression of DsbA. Addition of oxidized glutathione to the growth medium restores the wild-type phenotype in...

Topics

• Acid Phosphatase
• Disulfides
• Escherichia coli
• Genes
• In Vitro Techniques
• Phenotype
• Protein Disulfide Reductase (Glutathione)