Article
Towards a new interaction enzyme:coenzyme.
Biophysical chemistry - 1 Apr 2005
Martínez-Júlvez Marta, Tejero Jesús, Peregrina José R, Nogués Isabel, Frago Susana, Gómez-Moreno Carlos, Medina Milagros
Abstract excerpt
Ferredoxin-NADP(+) reductase catalyses NADP(+) reduction, being specific for NADP(+)/H. To understand coenzyme specificity determinants and coenzyme specificity reversion, mutations at the NADP(+)/H pyrophosphate binding and of the C-terminal regions have been simultaneously introduced in Anabaena FNR. The T155G/A160T/L263P/Y303S mutant was produced. The mutated enzyme presents similar k(cat) values for NADPH and...
Topics
- Anabaena
- Apoenzymes
- Ferredoxin-NADP Reductase
- Flavin-Adenine Dinucleotide
- Kinetics
- Mutation
- NADP
- Oxidation-Reduction
- Spectrum Analysis
- Tyrosine
