Article
Probing the role of lysines and arginines in the catalytic function of cytochrome P450d by site-directed mutagenesis. Interaction with NADPH-cytochrome P450 reductase.
The Journal of biological chemistry - 25 Feb 1991
Shimizu T, Tateishi T, Hatano M, Fujii-Kuriyama Y
Abstract excerpt
To identify amino acids of cytochrome P450d (P450d) which participate in the interaction with NADPH-cytochrome P450 reductase, we changed conserved ionic amino acids of P450d to others by site-directed mutagenesis. Turnover numbers (0.032-0.008 min-1) of purified mutants Lys94-Glu, Lys99-Glu, Lys105-Glu, Lys440-Glu, Lys453-Glu, Arg455-Glu, and Lys463-Glu toward 7-ethoxycoumarin were much lower than that (0.380...
Topics
- Amino Acid Sequence
- Arginine
- Cytochrome P-450 Enzyme System
- Lysine
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- NADPH-Ferrihemoprotein Reductase
- Saccharomyces cerevisiae
- Sequence Alignment