Article
Null thioredoxin and glutaredoxin Escherichia coli K-12 mutants have no enhanced sensitivity to mutagens due to a new GSH-dependent hydrogen donor and high increases in ribonucleotide reductase activity.
The Journal of biological chemistry - 17 Jun 1994
Miranda-Vizuete A, Martinez-Galisteo E, Aslund F, Lopez-Barea J, Pueyo C, Holmgren A
Abstract excerpt
This work investigates whether a mutator phenotype is associated to the simultaneous deficiency in thioredoxin and glutaredoxin, the two known hydrogen donors of ribonucleotide reductase. To this end, new Escherichia coli K-12 strains carrying delta trxA and/or grx::kan null mutations were constr...
Topics
- Bacterial Proteins
- Escherichia coli
- Genes, Bacterial
- Genotype
- Glutaredoxins
- Glutathione
- Mutagenesis
- Mutagenicity Tests
- Mutagens
- Oxidoreductases
- Proteins
- Ribonucleotide Reductases
- Species Specificity
- Thioredoxins