Article
Two additional glutaredoxins exist in Escherichia coli: glutaredoxin 3 is a hydrogen donor for ribonucleotide reductase in a thioredoxin/glutaredoxin 1 double mutant.
Proceedings of the National Academy of Sciences of the United States of America - 11 Oct 1994
Aslund F, Ehn B, Miranda-Vizuete A, Pueyo C, Holmgren A
Abstract excerpt
Thioredoxin (Trx) and glutaredoxin (Grx1) are hydrogen donors for ribonucleotide reductase, the key enzyme for deoxyribonucleotide biosynthesis. The viability of a double mutant lacking both Trx and Grx1 implies the presence of a third, unknown hydrogen donor. This paper reports the purification...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Chromatography, Affinity
- Chromatography, DEAE-Cellulose
- Chromatography, Gel
- Deoxyribonucleotides
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Glutaredoxins
- Kinetics
- Molecular Sequence Data
- Molecular Weight
- Mutation