Missense polymorphism (C/T224) in the human cathepsin D pro-fragment determined by polymerase chain reaction--single strand conformational polymorphism analysis and possible consequences in cancer cells.

Abstract excerpt

Overexpression of cathepsin D in human breast cancers is associated with a higher risk of relapse and metastasis. Also, pro-enzyme routing is altered in several tumoral mammary cell lines, leading to its hypersecretion. MCF7 cells compared to normal kidney carry a C-->T transition at position 224...

Topics

• Alleles
• Base Sequence
• Breast Neoplasms
• Cathepsin D
• Female
• Gene Frequency
• Humans
• Molecular Sequence Data
• Mutation
• Polymerase Chain Reaction
• Polymorphism, Genetic
• Tumor Cells, Cultured