Article
[A model prokaryotic promotor. II. Function of designs with mutations in the "-35" region].
Molekuliarnaia biologiia - 1 Jan 2000
Koroleva O N, Sergeev V N, Oretskaia T S, Drutsa V L
Abstract excerpt
A series of DNA duplexes corresponding to the E. coli consensus promoter with random hexanucleotides in the -35 region have been synthesized and characterized. The library of recombinant plasmids with synthetic promoter-like inserts, oriented in the direction of the marker gal operon of the initial vector, have been obtained. Analysis of the library on indicator medium and S1 mapping of the in vivo transcription...
Topics
- Base Sequence
- Escherichia coli
- Molecular Sequence Data
- Mutation
- Operon
- Plasmids
- Promoter Regions, Genetic
- Recombination, Genetic
- Transcription, Genetic