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Article

[Construction of a complete "library" of mutants at region -35 of a model prokaryotic promotor].

Bioorganicheskaia khimiia - 1 Aug 1990

Drutsa V L, Krivonos A V, Koroleva O N, Shabarova Z A

Abstract excerpt

A novel approach to study the variability of consensus sites of regulatory regions of DNA is proposed. The overall strategy includes chemical synthesis of representative series of all possible DNA structures under investigation, cloning and screening according to their function. The chemical-enzymatic synthesis of a complete library of 40-bp DNA duplexes, corresponding to the model prokaryotic promoter and...

Topics

Base Sequence
Chromatography, High Pressure Liquid
DNA
Electrophoresis, Polyacrylamide Gel
Gene Library
Molecular Sequence Data
Mutation
Nucleic Acid Heteroduplexes
Promoter Regions, Genetic
Regulatory Sequences, Nucleic Acid

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Sequence Homology, Nucleic Acid

Source

Identifiers

PMID: 2285423

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2285423-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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