Article
Promoters of the broad host range plasmid RK2: analysis of transcription (initiation) in five species of gram-negative bacteria.
Genetics - 1 Jan 1992
Greener A, Lehman S M, Helinski D R
Abstract excerpt
A broad host range cloning vector was constructed, suitable for monitoring promoter activity in diverse Gram-negative bacteria. This vector, derived from plasmid RSF1010, utilized the firefly luciferase gene as the reporter, since the assay for its bioluminescent product is sensitive, and measurements can be made without background from the host. Twelve DNA fragments with promoter activity were obtained from...
Topics
- Base Sequence
- Cloning, Molecular
- Genetic Vectors
- Gram-Negative Bacteria
- Luciferases
- Molecular Sequence Data
- Mutation
- Plasmids
- Promoter Regions, Genetic
- Transcription, Genetic