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Article

A base-paired hairpin structure essential for the functional priming signal for DNA replication of the broad host range plasmid RSF1010.

Nucleic acids research - 25 Oct 1993

Miao D M, Honda Y, Tanaka K, Higashi A, Nakamura T, Taguchi Y, Sakai H, Komano T, Bagdasarian M

Abstract excerpt

The two single-strand DNA initiation signals, ssiA(RSF1010) and ssiB(RSF1010) of the broad host-range plasmid RSF1010 contain proposed stem-loop structures. Nine single base-change mutations in the stem of the ssiA structure, each of which destroyed a relevant base pairing, damaged the ssiA activity. A second single-base change was introduced into each of the nine ssiA mutants in such a way that the base pairing...

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Identifiers

PMID: 8177737
DOI: 10.1093/nar/21.21.4900
PMCID: PMC311403

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Source receipt: pubmed, retrieved 7 Sept 2026.

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