Article
Resonance Raman spectroscopic studies of ligated mutant myoglobins.
Biochimica et biophysica acta - 16 Feb 1994
Biram D, Hester R E
Abstract excerpt
Ligand binding (CO and N3-) to wild-type porcine myoglobin and to several mutant forms, expressed and purified from E. coli cells, has been studied using Raman spectroscopy. The v(Fe-CO) stretching vibration in MbIICO has been compared for the wild-type and mutant proteins. This gives a broad band consisting of five components, indicating five possible configurations of the bound CO. The distal pocket mutants...
Topics
- Animals
- Metmyoglobin
- Mutation
- Myoglobin
- Spectrum Analysis, Raman
- Swine