Article
Alteration of axial coordination by protein engineering in myoglobin. Bisimidazole ligation in the His64-->Val/Val68-->His double mutant.
The Journal of biological chemistry - 7 Jul 1995
Dou Y, Admiraal S J, Ikeda-Saito M, Krzywda S, Wilkinson A J, Li T, Olson J S, Prince R C, Pickering I J, George G N
Abstract excerpt
Pig and human myoglobin have been engineered to reverse the positions of the distal histidine and valine (i.e. His64(E7)-->Val and Val68(E11)-->His). Spectroscopic and ligand binding properties have been measured for human and pig H64V/V68H myoglobin, and the structure of the pig H64V/V68H double...
Topics
- Animals
- Carbon Monoxide
- Crystallography, X-Ray
- Electron Spin Resonance Spectroscopy
- Heme
- Histidine
- Humans
- Imidazoles
- Ligands
- Models, Molecular
- Mutation
- Myoglobin
- Protein Engineering
- Spectrophotometry