DNA sequence divergence among derivatives of Escherichia coli K-12 detected by arbitrary primer PCR (random amplified polymorphic DNA) fingerprinting.

Abstract excerpt

Derivatives of Escherichia coli K-12 of known ancestry were characterized by random amplified polymorphic DNA (RAPD) fingerprinting to better understand genome evolution in this family of closely related strains. This sensitive method entails PCR amplification with arbitrary primers at low stringency and yields arrays of anonymous DNA fragments that are strain specific. Among 150 fragments scored, eight were...

Topics

• Base Sequence
• Biological Evolution
• Blotting, Southern
• Chromosomes, Bacterial
• DNA Fingerprinting
• DNA, Bacterial
• Escherichia coli
• Molecular Sequence Data
• Mutation
• Nucleic Acid Hybridization
• Plasmids