Article
Exploration of biases that affect the interpretation of restriction fragment patterns produced by pulsed-field gel electrophoresis.
Journal of clinical microbiology - 1 Dec 2004
Singer Randall S, Sischo William M, Carpenter Tim E
Abstract excerpt
Pulsed-field gel electrophoresis (PFGE) has been used extensively in epidemiological investigations of bacteria, especially during food-borne outbreaks or nosocomial infections. The relationship between similarities in PFGE patterns and true genetic relatedness is poorly understood. In this study, computer-simulated populations of Escherichia coli isolates were created by mutating the sequence of E. coli K-12...
Topics
- Bacterial Typing Techniques
- Bias
- Computer Simulation
- DNA Restriction Enzymes
- DNA, Bacterial
- Data Interpretation, Statistical
- Disease Outbreaks
- Electrophoresis, Gel, Pulsed-Field
- Escherichia coli Infections
- Escherichia coli K12