Article
Inhibition of the synthesis of a cytochrome-c-oxidase subunit isoform by antisense RNA.
European journal of biochemistry - 1 Feb 1994
Sandonà D, Bisson R
Abstract excerpt
To investigate the role of subunit VIIe, an oxygen-regulated subunit isoform of Dictyostelium discoideum cytochrome-c oxidase, the full-length cDNA was inserted into an expression vector under the control of an actin promoter in the sense and antisense orientation. The DNA constructs were used for stable transformation of the slime mold amoebae. In most of the 28 antisense clones tested, the concentration of...
Topics
- Animals
- Blotting, Southern
- Cloning, Molecular
- Culture Media
- DNA, Complementary
- Dictyostelium
- Electron Transport Complex IV
- Electrophoresis, Polyacrylamide Gel
- Mutation
- Oxygen
- RNA, Antisense