Article
Use of PCR for the determination of the frequency of the delta F508 mutation in Brazilian cystic fibrosis patients.
Memorias do Instituto Oswaldo Cruz - 1 Jan 2000
de Miranda A B, Llerena Júnior J, Dallalana L T, Moura-Neto R S, Suffys P N, Degrave W M
Abstract excerpt
The delta F508 mutation in the cystic fibrosis (CF) gene was studied in a population of 18 Brazilian CF patients and their 17 families by use of PCR and differential hybridization with oligonucleotides. In a total of 34 chromosomes considered, 12 (35%) carried the F508 deletion, a frequency much...
Topics
- Base Sequence
- Brazil
- Chromosomes, Human, Pair 7
- Cystic Fibrosis
- Female
- Gene Frequency
- Humans
- Male
- Molecular Sequence Data
- Mutation
- Pedigree
- Polymerase Chain Reaction