Article
Involvement of FtsH in protein assembly into and through the membrane. I. Mutations that reduce retention efficiency of a cytoplasmic reporter.
The Journal of biological chemistry - 18 Feb 1994
Akiyama Y, Ogura T, Ito K
Abstract excerpt
To identify cellular factors that assist in membrane protein biogenesis, we looked for mutants affected in the "stop transfer" anchoring process. Using a SecY-PhoA fusion protein in which alkaline phosphatase (PhoA) mature sequence is attached to the last cytoplasmic domain following the 10th tra...
Topics
- ATP-Dependent Proteases
- Adenosine Triphosphatases
- Alkaline Phosphatase
- Bacterial Proteins
- Base Sequence
- Cell Membrane
- DNA Primers
- Escherichia coli
- Escherichia coli Proteins
- Genes, Bacterial
- Genotype
- Membrane Proteins
- Methylnitronitrosoguanidine
- Molecular Sequence Data
- Mutagenesis
- Phenotype
- Plasmids
- Polymerase Chain Reaction