A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers.

doi:10.1046/j.1365-313x.1993.04020403.x

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Article

A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers.

The Plant journal : for cell and molecular biology - 1 Aug 1993

Konieczny A, Ausubel F M

Abstract excerpt

A set of mapping markers have been designed for Arabidopsis thaliana that correspond to DNA fragments amplified by the polymerase chain reaction (PCR). The ecotype of origin of these amplified fragments can be determined by cleavage with a restriction endonuclease. Specifically, 18 sets of PCR pr...

Topics

Arabidopsis
Base Sequence
Chromosome Mapping
DNA
DNA Restriction Enzymes
Genes, Plant
Genetic Markers
Molecular Sequence Data
Mutation
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length

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