Article
A PCR plus restriction enzyme-based technique for detecting target-enzyme mutations at position Pro-106 in glyphosate-resistant Lolium perenne.
PloS one - 1 Jan 2021
Ghanizadeh Hossein, Griffiths Andrew G, Buddenhagen Christopher E, Anderson Craig B, Harrington Kerry C
Abstract excerpt
The first step in managing herbicide-resistant weeds is to confirm their resistance status. It is, therefore, crucial to have a rapid, reliable and cost-effective technique to assess samples for herbicide resistance. We designed and evaluated three derived cleaved amplified polymorphic sequence (dCAPS) markers for detecting glyphosate resistance in Lolium perenne. conferred by non-synonymous mutations at...
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