Article
Temperature-sensitive lesions in the capsid proteins of the rotavirus mutants tsF and tsG that affect virion assembly.
Virology - 1 Oct 1994
Mansell E A, Ramig R F, Patton J T
Abstract excerpt
The SA11 rotavirus mutants tsF and tsG contain temperature sensitive (ts) lesions in the capsid proteins VP2 and VP6, respectively, that interfere with their ability to assemble. To understand the nature of their lesions, full-length cDNAs of tsF gene 2 and tsG gene 6 were prepared from viral mRNA by reverse transcription and polymerase chain reaction. Comparative sequence analysis indicated that the ts phenotype...
Topics
- Amino Acid Sequence
- Antigens, Viral
- Base Sequence
- Capsid
- Capsid Proteins
- Cell Line
- DNA, Complementary
- Inclusion Bodies, Viral
- Molecular Sequence Data
- Mutation
- Myristic Acid