Article
Human herpesvirus 6B origin: sequence diversity, requirement for two binding sites for origin-binding protein, and enhanced replication from origin multimers.
Journal of virology - 1 Oct 1994
Dewhurst S, Krenitsky D M, Dykes C
Abstract excerpt
A previously identified human herpesvirus 6B (HHV-6B) origin of DNA replication contains two binding sites for the origin-binding protein (OBPH6B). We have investigated the functional significance of these sites by determining the replication efficiencies of mutated origin sequences, using a transient replication assay. The results indicate that both sites are required for DNA replication. In addition, we have...
Topics
- Base Sequence
- Binding Sites
- DNA Primers
- DNA Replication
- DNA, Viral
- DNA-Binding Proteins
- Genetic Variation
- Herpesvirus 6, Human
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction