Article
A microtiter plate assay for determining apolipoprotein E genotype and discovery of a rare allele.
Human mutation - 1 Jan 1994
Livak K J, Hainer J W
Abstract excerpt
Genotype determination using the solid-phase minisequencing method of Syvänen et al. (1990, 1993) has been adapted for use with fluorescein-labeled dideoxynucleotides (F-ddNTPs). PCR is performed using one biotinylated primer and one unbiotinylated primer. The biotinylated products are captured in streptavidin-coated microtiter wells. Following removal of nonbiotinylated strands with NaOH, the bound strands are...
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