Article
A microtiter plate assay for determining apolipoprotein E genotype and discovery of a rare allele.
Human mutation - 1 Jan 1994
Livak K J, Hainer J W
Abstract excerpt
Genotype determination using the solid-phase minisequencing method of Syvänen et al. (1990, 1993) has been adapted for use with fluorescein-labeled dideoxynucleotides (F-ddNTPs). PCR is performed using one biotinylated primer and one unbiotinylated primer. The biotinylated products are captured i...
Topics
- Alleles
- Apolipoproteins E
- Bacterial Proteins
- Base Sequence
- Biotin
- DNA Primers
- Deoxyribonucleotides
- Enzyme-Linked Immunosorbent Assay
- Fluoresceins
- Fluoroimmunoassay
- Genotype
- Humans
- Male