[Quantitative analysis of hepatitis C virus RNA by competitive nested PCR].

Article

[Quantitative analysis of hepatitis C virus RNA by competitive nested PCR].

Nihon rinsho. Japanese journal of clinical medicine - 1 Jul 1994

Kobayashi M

Abstract excerpt

A method that allows the quantitation of hepatitis C virus (HCV) RNA is described. The RNA was extracted from serum sample and reverse transcribed. Target cDNA was then co-amplified by nested polymerase chain reaction with a known amount of competitive template at various concentrations. Since th...

Topics

Genotype
Hepacivirus
Hepatitis C
Humans
Nucleic Acid Hybridization
Polymerase Chain Reaction
RNA, Viral

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