Article
[Quantitative analysis of hepatitis C virus RNA by competitive nested PCR].
Nihon rinsho. Japanese journal of clinical medicine - 1 Jul 1994
Kobayashi M
Abstract excerpt
A method that allows the quantitation of hepatitis C virus (HCV) RNA is described. The RNA was extracted from serum sample and reverse transcribed. Target cDNA was then co-amplified by nested polymerase chain reaction with a known amount of competitive template at various concentrations. Since this internal control DNA uses the same primers as those of the target and is distinguishable from the target cDNA after...
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