Article
Development and evaluation of a quantitative competitive reverse transcription polymerase chain reaction (RT-PCR) for hepatitis C virus RNA in serum using transcribed thio-RNA as internal control.
Journal of virological methods - 1 Mar 2004
Hazari Sidhartha, Acharya Subrat Kumar, Panda Subrat Kumar
Abstract excerpt
A method for quantitation of hepatitis C virus (HCV) RNA was developed based on competitive reverse transcription polymerase chain reaction (RT-PCR) using in vitro transcribed mutated thio-RNA as a competitor template. The thio-RNA is more resistant to RNAse and is stable over a year. This assay was compared with the commercially available Roche Amplicor HCV Monitor assay V 2.0 and real time PCR using SYBR green...
Topics
- Genotype
- Hepacivirus
- Hepatitis C, Chronic
- Humans
- RNA, Viral
- Reproducibility of Results
- Reverse Transcriptase Polymerase Chain Reaction
- Sensitivity and Specificity
- Viral Load
- Viremia