Article
Quantitative Competitive Reverse Transcription-PCR for Quantification of Dengue Virus RNA
1 Jan 2000
Abstract excerpt
A quantitative competitive reverse transcription-PCR assay was developed to quantify dengue virus RNA in this study. The main features include a primer pair targeting a highly conserved region in the capsid and the addition of competing RNA that contains an internal deletion to provide a stringent internal control for quantification. It can be utilized to quantify RNA isolated from the four dengue virus serotypes...