Article
Establishment of human parotid pleomorphic adenoma cells in culture: morphological and biochemical characterization.
In vitro cellular & developmental biology. Animal - 1 May 1994
Prasad K N, Carvalho E, Edwards-Prasad J, La Rosa F G, Kumar S, Kim J H, Meyers A, Kentroti S
Abstract excerpt
This study reports the establishment of alpha-amylase-producing human parotid pleomorphic adenoma cell lines (2HP and 2HP1) which have been maintained in culture for over 1 yr. The procedures required preparation of cellular clumps from tumor tissue and plating them on plasma clot or precoated di...
Topics
- Adenoma, Pleomorphic
- Adult
- Animals
- Blotting, Western
- Cell Division
- Culture Media
- Fluorescent Antibody Technique
- Humans
- Male
- Mice
- Mice, Nude
- Neoplasm Transplantation
- Parotid Neoplasms
- Phenotype
- RNA, Messenger
- Tumor Cells, Cultured
- alpha-Amylases