Article
Establishment of human parotid pleomorphic adenoma cells in culture: morphological and biochemical characterization.
In vitro cellular & developmental biology. Animal - 1 May 1994
Prasad K N, Carvalho E, Edwards-Prasad J, La Rosa F G, Kumar S, Kim J H, Meyers A, Kentroti S
Abstract excerpt
This study reports the establishment of alpha-amylase-producing human parotid pleomorphic adenoma cell lines (2HP and 2HP1) which have been maintained in culture for over 1 yr. The procedures required preparation of cellular clumps from tumor tissue and plating them on plasma clot or precoated dishes. During the initial phase of growth they required modified MCDB-153 medium without serum. When cells showed signs...
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