Requirement of a COOH-terminal pro-sequence for the extracellular secretion of aqualysin I (a thermophilic subtilisin-type protease) in Thermus thermophilus.

doi:10.1111/j.1574-6968.1994.tb07009.x

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Article

Requirement of a COOH-terminal pro-sequence for the extracellular secretion of aqualysin I (a thermophilic subtilisin-type protease) in Thermus thermophilus.

FEMS microbiology letters - 1 Jul 1994

Lee Y C, Koike H, Taguchi H, Ohta T, Matsuzawa H

Abstract excerpt

Thermus thermophilus cells harboring an expression plasmid for the aqualysin I gene secrete the mature enzyme into the medium. In an Escherichia coli expression system, a precursor of the enzyme with the C-terminal pro-sequence is accumulated in the cells, and upon treatment at 65 degrees C the a...

Topics

Amino Acid Sequence
Enzyme Precursors
Gene Deletion
Genes, Bacterial
Mutation
Plasmids
Serine Endopeptidases
Thermus thermophilus
Transformation, Bacterial

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