Article
Cloning of ornithine delta-aminotransferase cDNA from Vigna aconitifolia by trans-complementation in Escherichia coli and regulation of proline biosynthesis.
The Journal of biological chemistry - 5 Sept 1993
Delauney A J, Hu C A, Kishor P B, Verma D P
Abstract excerpt
Proline prototrophy was restored to an Escherichia coli proBA proline auxotroph by ornithine and a mothbean (Vigna aconitifolia) cDNA expression library. This novel strategy, "trans-complementation," allowed isolation of a cDNA encoding ornithine delta-aminotransferase (delta-OAT). This enzyme tr...
Topics
- 1-Pyrroline-5-Carboxylate Dehydrogenase
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA
- Escherichia coli
- Fabaceae
- Glutamates
- Glutamic Acid
- Kinetics
- Molecular Sequence Data
- Mutation
- Nitrogen
- Ornithine-Oxo-Acid Transaminase
- Osmotic Pressure
- Oxidoreductases Acting on CH-NH Group Donors
- Plants
- Plants, Medicinal