Article
Cloning and characterization of a cDNA encoding aspartate aminotransferase-P1 from Lupinus angustifolius root tips.
Plant physiology - 1 Feb 1994
Winefield C S, Reddington B D, Jones W T, Reynolds P H, Farnden K J
Abstract excerpt
A root tip cDNA library, constructed in the lambda Zap II expression vector, was immunoscreened with a monoclonal antibody raised against aspartate aminotransferase-P1 from Lupinus angustifolius L. var Uniharvest. One 1452-base pair clone was isolated. The encoded protein sequence had high homolo...
Topics
- Amino Acid Sequence
- Animals
- Aspartate Aminotransferases
- Base Sequence
- Cloning, Molecular
- DNA
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Genetic Complementation Test
- Molecular Sequence Data
- Mutation
- Plants
- Recombinant Proteins
- Saccharomyces cerevisiae
- Sequence Homology, Amino Acid