Article
Determination of the carbon monoxide binding constants of myoglobin mutants: comparison of kinetic and equilibrium methods.
Biochemistry - 12 Jul 1994
Balasubramanian S, Lambright D G, Simmons J H, Gill S J, Boxer S G
Abstract excerpt
The carbon monoxide (CO) binding constants of human myoglobin (Mb) and several single-site mutants have been determined using two different methods. In the kinetic method, which is commonly used for this ligand, the overall association (k(on)) and dissociation (k(off)) rates of CO were measured b...
Topics
- Binding Sites
- Carbon Monoxide
- Humans
- Kinetics
- Mutagenesis, Site-Directed
- Mutation
- Myoglobin
- Nitric Oxide
- Photolysis
- Protein Conformation
- Spectroscopy, Fourier Transform Infrared
- Structure-Activity Relationship