Article
Detection of the homozygous recessive genotype for deficiency of uridine monophosphate synthase by DNA typing among bovine embryos produced in vitro.
Journal of reproduction and fertility - 1 Mar 1994
Schwenger B, Tammen I, Aurich C
Abstract excerpt
A PCR-based DNA test for the genetic defect resulting in the deficiency of uridine monophosphate synthase (DUMPS) was developed for embryonic cells to identify the homozygous recessive genotype. This genotype is usually lethal at about day 40 of gestation. Oocytes from a cow, heterozygous for DUMPS, were fertilized in vitro with spermatozoa from a DUMPS-carrier bull and cultured in vitro until the morula or...
Topics
- Animals
- Base Sequence
- Cattle
- Cattle Diseases
- DNA
- DNA Primers
- Fertilization in Vitro
- Genes, Recessive
- Genotype
- Molecular Sequence Data
- Multienzyme Complexes