Article
Two genetically-distinct and differentially-regulated aconitases (AcnA and AcnB) in Escherichia coli.
Microbiology (Reading, England) - 1 Oct 1994
Gruer M J, Guest J R
Abstract excerpt
An acnA mutant of Escherichia coli was constructed by replacing the chromosomal acnA gene by an internally deleted derivative containing a kanR cassette. Southern and Western blotting confirmed that the acnA gene had been replaced by the disrupted gene and that the aconitase A protein was no longer expressed. However, the mutant failed to exhibit the anticipated glutamate auxotrophy and it retained a residual...
Topics
- Aconitate Hydratase
- Blotting, Southern
- Blotting, Western
- Citric Acid Cycle
- Cloning, Molecular
- Culture Media
- Escherichia coli
- Fumarate Hydratase
- Gene Expression Regulation, Bacterial
- Mutation