Article
Construction and properties of aconitase mutants of Escherichia coli.
Microbiology (Reading, England) - 1 Jun 1997
Gruer Megan J, Bradbury Alan J, Guest John R
Abstract excerpt
Escherichia coli contains two genes (acnA and acnB) encoding aconitase activities. An acnB mutant was engineered by replacing the chromosomal acnB gene by an internally deleted derivative containing a tetR cassette. An acnB double mutant was then made by transducing a previously constructed acnA:...
Topics
- Aconitate Hydratase
- Cloning, Molecular
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Gene Expression Regulation, Enzymologic
- Genes, Bacterial
- Isoenzymes
- Mutation