Article
Parasexual crosses and hybrid selection with a near haploid variant of the epithelioma papulosum cyprini, cell line EPC: a tissue culture model for the analyses of dominance, recessiveness, and complementation of mutant phenotypes.
Molecular marine biology and biotechnology - 1 Aug 1994
Li Z F, Murphy T, Gao D, Ellis D, Sauerbier A, Sauerbier W
Abstract excerpt
We describe techniques for insertional mutagenesis of tissue-cultured piscine cells in which we use transfection with G418 and hygromycin B resistance-conferring plasmids, cell matings by electrofusion, and positive selections of fusion hybrids by dual challenge with the antibiotics G418 and hygr...
Topics
- Animals
- Carps
- Cell Division
- Cell Fusion
- Cell Line
- Clone Cells
- Cloning, Molecular
- Contact Inhibition
- Crosses, Genetic
- Drug Resistance
- Genes, Dominant
- Genes, Recessive
- Genetic Complementation Test
- Gentamicins
- Hygromycin B