Article
Effect on thermostability and catalytic activity of introducing disulfide bonds into Aspergillus awamori glucoamylase.
Protein engineering - 1 Aug 1998
Li Y, Coutinho P M, Ford C
Abstract excerpt
Two additional disulfide bonds and three combined thermostabilizing mutations were introduced into Aspergillus awamori glucoamylase to test their effects on enzyme thermostability and catalytic properties. The single cysteine mutations N20C, A27C, T72C and A471C were made and combined to produce...
Topics
- Aspergillus
- Disulfides
- Enzyme Activation
- Enzyme Stability
- Glucan 1,4-alpha-Glucosidase
- Hot Temperature
- Models, Molecular
- Mutation
- Protein Conformation
- Protein Engineering
- Recombinant Proteins