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Application of spheroid culture to human hepatocytes and maintenance of their differentiation

1 Jan 1994

Abstract excerpt

Human hepatocytes cultured with a hormonally defined medium on non-adherent poly-(2-hydroxyethyl methacrylate)-coated surface were able to form spheroids. The maintenance of liver-specific functions was assessed by following secretion of albumin, transferrin and alpha 1-antitrypsin that were still detectable after 4 months of spheroidal culture. Moreover, cytochrome P-450 IA was induced by methylcholanthrene for...

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PMID: 7987245
DOI: 10.1016/0248-4900(94)90058-2

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