Article
Isolation and characterization of the gene conferring thiamine-inducible expression from Saccharomyces cerevisiae.
Bioscience, biotechnology, and biochemistry - 1 Aug 1999
Shiba Y, Ono C, Ichikawa K, Serizawa N, Yoshikawa H
Abstract excerpt
The production level of CPY in Saccharomyces cerevisiae KS58-2D/pCY303 was drastically decreased when thiamine was not added to the culture medium. We isolated and characterized the mutants that could produce CPY even though thiamine was absent from the medium. Using complementation screening in the mutants obtained, we isolated a gene that was involved in the thiamine-inducible expression, TIE1, which...
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