Article
An improved fragile X Southern blot probe without the CGGs eliminates background bands.
Molecular and cellular probes - 1 Jun 1994
Davidow L S, Barberio D, Cao J, McCormick I
Abstract excerpt
The largest of the commonly used probes for Southern blot diagnosis of fragile X mental retardation syndrome spans the CGG repeat cluster in the FMR-1 gene. This probe causes the appearance of 'common' or 'constant' background bands which occasionally complicate the interpretation of autoradiogra...
Topics
- Autoradiography
- Blotting, Southern
- Cytosine Nucleotides
- DNA
- Fragile X Syndrome
- Guanine Nucleotides
- Humans
- Mutation
- Polymerase Chain Reaction